Serology Frequently Asked Questions
Welcome to the Auburn University Serology-Virology Laboratory Frequently Asked Questions page. This resource provides guidance on specimen collection and submission, shipping requirements, test options, turnaround times, and result reporting. Our goal is to help navigate the submission process efficiently and ensure the highest quality diagnostic testing services for their patients and clients. Reviewing this information before submitting samples can help ensure timely processing and reporting of results.
To help us respond as quickly as possible, please email rabies@auburn.edu.
FAVN Testing FAQs
- What forms are required?
FAVN
Expedited FAVN
NOTE: A printed copy of the FAVN Form must be included in the shipment.
You will need to register for our online portal to access the Fillable FAVN Form.
- What is the veterinarian responsible for?
The submitting veterinarian is responsible for:
- Accurate completion of forms
- Sample collection
- Verification of microchip information (FAVN testing)
- Scan and verify the microchip immediately before blood collection.
- Ensure the microchip number on the submission form exactly matches the scanned number.
- Verify the microchip number matches all travel documentation.
- Ensuring all required information is included prior to submission
Incomplete forms may delay testing.
The submitting clinic is considered our client and is responsible for all submissions and charges.
Sample Collection and Handling
- What sample type is required?
FAVN and Expedited FAVN: Serum is required.
- Are separator tubes acceptable?
Yes. However, transferring serum into a non-additive tube prior to shipment is recommended.
Each sample must be labeled with both the animal’s name and microchip number. Tubes that are inappropriately labeled are cancelled without exception.
- What is the minimum sample volume?
FAVN and RFFIT testing: 1.0–2.0 mL serum recommended.
- Is fasting required?
Not routinely, but an 8–12 hour fast may reduce lipemia.
- What are the sample storage recommendations?
- Refrigerate: 7–10 days
- Freeze at -20°C for long-term storage
- What about hemolyzed or lipemic samples?
Mild hemolysis/lipemia may be acceptable. A simple assessment is to hold the serum tube up to printed text; if the print is visible through the sample, it is generally acceptable for testing.
A toxic result indicates that the submitted sample adversely affected the cell culture system used for testing, preventing accurate interpretation. This may occur with severely hemolyzed, contaminated, lipemic, or improperly handled specimens.
Packaging and Shipping
Please refer to the packaging and shipping guidelines found on Sample Submissions and Shipping.
Results
Samples submitted with the online fillable form:
- The official results are digital, and no hard copies are distributed. An emailed copy of the results will be sent to the submitting clinic. Quarantine officials will scan the QR code on the FAVN Report for result verification.
Samples submitted with a paper submission form:
- The rabies antibody titer results are printed on an adhesive sticker and placed on a copy of the “FAVN REPORT FORM” that was submitted with the sample. This is the official FAVN report.
- Who receives the results?
-
Results are emailed to the submitting veterinary clinic.
Certain destinations also receive reports directly from the laboratory.
Report Amendments, Reissues and Test Cancellations
- How can amendments be requested?
- The submitting clinic needs to email the laboratory directly at rabies@auburn.edu for any requested amendments.
- Amendments are reviewed on a case-by-case basis and according to laboratory policy.
- How are reissued reports requested?
Requests must be submitted by the submitting veterinary clinic via the Pathobiology Diagnostic Lab Portal.
- How do I cancel my test?
All cancellation requests must be made via email from the submitting clinic directly to the laboratory at rabies@auburn.edu before the sample is processed for testing.
Other Serologies
RFFIT Testing Information
- What is the difference between RFFIT screen and endpoint?
- Endpoint: quantitative titer
- Screen: qualitative positive/negative threshold
- Can RFFIT be used for international pet travel?
No. FAVN is required for rabies-free destinations.
- What is a passing RFFIT titer?
Many authorities consider ≥ 0.50 IU/mL acceptable for presumed protection.
General Serology Testing
- What is the difference between screening and endpoint?
- Endpoint: exact titer
- Screen: positive/negative threshold
- What are the other available serology assays?
Refer to:
- What are the specimen requirements?
Requirements vary by assay. Consult the online catalog prior to submission.
Please contact the lab directly regarding CSF sample submissions.
